[1]王锦锋,陆海峰,王忠,等.5个蝙蝠蛾拟青霉菌株的对比筛选[J].亚热带农业研究,2020,16(01):47-51.[doi:10.13321/j.cnki.subtrop.agric.res.2020.01.009]
 WANG Jinfeng,LU Haifeng,WANG Zhong,et al.Comparative screening of five Paecilomyces hepiali strains[J].,2020,16(01):47-51.[doi:10.13321/j.cnki.subtrop.agric.res.2020.01.009]
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5个蝙蝠蛾拟青霉菌株的对比筛选()
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《亚热带农业研究》[ISSN:1006-6977/CN:61-1281/TN]

卷:
16
期数:
2020年01期
页码:
47-51
栏目:
出版日期:
2020-05-01

文章信息/Info

Title:
Comparative screening of five Paecilomyces hepiali strains
作者:
王锦锋 陆海峰 王忠 吴鸿雪
安发福建生物科技有限公司/福建省天然生物活性物质企业重点实验室, 福建 宁德 352000
Author(s):
WANG Jinfeng LU Haifeng WANG Zhong WU Hongxue
Alpha Fujian Bio-technology Co., Ltd. /Key Laboratory of Natural Bioactive Substances of Fujian Province, Ningde, Fujian 352000, China
关键词:
蝙蝠蛾拟青霉菌丝干重腺苷含量甘露醇含量
Keywords:
Paecilomyces hepialimycelialdry weightadenosine contentmannitol content
分类号:
S567.3
DOI:
10.13321/j.cnki.subtrop.agric.res.2020.01.009
摘要:
[目的] 比较3.1487(T1)、3.1772(T2)、5494(T3)、B01461(T4)和BN339916(T5)等5个蝙蝠蛾拟青霉菌株的优劣。[方法] 通过比较5个菌株的菌丝干重与腺苷、甘露醇、粗多糖、蛋白质、灰分等含量,筛选优良菌株。[结果] 菌丝干重以T1菌株最高,比最低的T2菌株高10%,但与其他菌株无显著差异;腺苷含量以T5菌株最高,是药典标准含量的1.45倍,显著高于其他4个菌株(P<0.05);甘露醇含量以T1菌株最高,是药典标准含量的1.54倍,但5个菌株之间无显著差异;粗多糖和蛋白质含量均以T5菌株最高,T2菌株最低;灰分含量以T2菌株最高,T3菌株最低。[结论] 灰色关联度分析表明,T5菌株综合表现最优,可作为企业生产的优良菌株。
Abstract:
[Purpose] To compare the advantages and disadvantages of five Paecilomyces hepiali strains, namely, 3.1487 (T1), 3.1772 (T2), 5494 (T3), B01461(T4), and BN339916 (T5).[Method] The mycelium dry weight, adenosine content, mannitol content, contents of crude polysaccharide, protein, ash, and others of five P.hepiali strains were compared for screening out the superior strains.[Result] T1 strain had the highest mycelial dry weight, which was 10% higher than the lowest mycelial dry weight in T2 strain, but was not significantly different than the mycelial dry weight of other three strains. The adenosine content of T5 strain was the highest, which was 1.45 times of the standard content of the Pharmacopoeia and significantly higher than the other four strains (P<0.05). The mannitol content was the highest in T1 strain, which was 1.54 times of the standard content of the Pharmacopoeia, but there was no significant difference among the five strains. The highest crude polysaccharide and protein contents were in T5 strain and the lowest were in T2. T3 strain had the lowest ash content and T2 strain had the highest ash content.[Conclusion] Comprehensive analysis by grey correlation degree method showed that T5 strain had the best comprehensive performance and could be used as an excellent strain for production.

参考文献/References:

[1] 戴如琴,兰江丽,陈伟华,等.蝙蝠蛾拟青霉新种的研究[J].北京农业大学学报,1989,15(2):221-224.
[2] 戴如琴,李晓明,邵爱娟,等. 蝙蝠蛾拟青霉名称的合格化[J].菌物学报,2008,27(5):641-644.
[3] WU Z W, LU J W, WANG X Q, et al. Optimization for production of exopolysaccharides with antitumor activity in vitro from Paecilomyces hepiali[J]. Carbohydrate Polymers, 2014,99:226-234.
[4] 吕敏,吕圭源.虫草菌粉中2种菌丝体的化学成分和药理活性研究进展[J].中草药,2017,48(22):4791-4796.
[5] 张洁宏,赵鹏,李彬,等.蝙蝠蛾拟青霉菌丝体增强免疫力功能动物试验研究[J].广西医科大学学报,2013,30(4):520-522.
[6] 刘俊,杨本寿,姜国银.蝙蝠蛾拟青霉的抑菌活性与菌丝体发酵化学组分的研究[J].昆明医学院学报,2012,33(6):32-34,38.
[7] HU W J, WANG J, GUO W Y, et al. Studies on characteristics and anti-diabetic and -nephritic effects of polysaccharides isolated from Paecilomyces hepiali fermentation mycelium in db/db mice[J]. Carbohydrate Polymers, 2020,232:115766.
[8] 曹阳,张淑华,梁冲,等.蝙蝠蛾拟青霉菌丝体化学成分研究[J].中国药物化学杂志,2019,29(4):300-304.
[9] 周娴,黄梦琦,邱孺,等.冬虫夏草组成及生产应用研究进展[J].民营科技,2018(9):98-99.
[10] 张倩,吴海亮,于士军.蝙蝠蛾拟青霉高产腺苷液体培养基优化[J].安徽农学通报,2010,16(24):25-27,70.
[11] 洪露露.蝙蝠蛾拟青霉虫草素高产菌株的选育及其培养条件优化[D].杭州:浙江工业大学,2019.
[12] 张命龙,焦春伟,谢意珍,等.蝙蝠蛾拟青霉液体发酵工艺优化及菌丝体腺苷含量的检测[J].菌物学报,2016,35(2):188-198.
[13] 王锦锋,王忠,吴鸿雪.中国被毛孢高产孢菌株的筛选[J].亚热带农业研究,2019,15(2):132-137.
[14] 姜磊.蝙蝠蛾拟青霉(Paecilomyces hepiali)菌丝体化学成分和药理活性的研究[D].长春:吉林农业大学,2011.
[15] 周毓麟,李兰洲,胡文继,等.蝙蝠蛾拟青霉质量标准及免疫活性研究[J].中南民族大学学报(自然科学版),2017,36(1):24-27.
[16] 中华人民共和国农业部.食用菌中粗多糖含量的测定:SN/T 1676—2008[S].北京:中国农业出版社,2008.
[17] 中华人民共和国卫生部,中国国家标准化管理委员会.食品中蛋白质的测定:GB/T 5009.5—2003[S].北京:中国标准出版社,2003.
[18] 中华人民共和国卫生部.食品中灰分的测定:GB 5009.4—2010[S].北京:中国标准出版社,2010.
[19] 刘录祥.灰色系统理论应用于作物新品种综合评估初探[J].中国农业科学,1989,22(3):22-24.
[20] 国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:172-1074.
[21] 董彩虹,李文佳,李增智,等.我国虫草产业发展现状、问题及展望——虫草产业发展金湖宣言[J].菌物学报,2016,35(1):1-15.

备注/Memo

备注/Memo:
收稿日期:2020-01-02。
作者简介:王锦锋(1990-),男,硕士。研究方向:食药用菌。Email:535108335@qq.com。
更新日期/Last Update: 1900-01-01