[1]夏科,吴巧芬,赵志国,等.石斛属植物再生及遗传转化研究进展[J].亚热带农业研究,2020,16(02):110-117.[doi:10.13321/j.cnki.subtrop.agric.res.2020.02.008]
 XIA Ke,WU Qiaofen,ZHAO Zhiguo,et al.Research progress on plant regeneration and genetic transformation of the Dendrobium genus[J].,2020,16(02):110-117.[doi:10.13321/j.cnki.subtrop.agric.res.2020.02.008]
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石斛属植物再生及遗传转化研究进展()
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《亚热带农业研究》[ISSN:1006-6977/CN:61-1281/TN]

卷:
16
期数:
2020年02期
页码:
110-117
栏目:
出版日期:
2020-07-22

文章信息/Info

Title:
Research progress on plant regeneration and genetic transformation of the Dendrobium genus
作者:
夏科 吴巧芬 赵志国 蒋庆鸿 仇硕
广西壮族自治区中国科学院广西植物研究所, 广西 桂林 541006
Author(s):
XIA Ke WU Qiaofen ZHAO Zhiguo JIANG Qinghong QIU Shuo
Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi 541006, China
关键词:
石斛属组织培养再生体系遗传转化
Keywords:
Deniudrobmtissue cultureregeneration systemgenetic transformation
分类号:
Q943.2
DOI:
10.13321/j.cnki.subtrop.agric.res.2020.02.008
摘要:
[目的] 了解石斛属植物再生及遗传转化研究现状,为石斛属植物分子生物学相关研究提供技术支撑。[方法] 通过文献检索,综述了石斛属植物的组培再生和遗传转化研究现状、存在问题及其产业化应用现状。[结果] 石斛属多个种的组培再生体系及产业化推广比较完善;但大部分种类遗传转化过程中仍存在转化效率低及很难获得转基因植株等问题。[结论] 应进一步完善农杆菌介导转化法,并将其与基因枪法相结合;应探索其他植物遗传转化方法。
Abstract:
[Purpose] To understand the current research status of plant regeneration and genetic transformation in Dendrobium species, and to provide technical support for the research related to the molecular biology of the Dendrobium genus.[Method] Through literature search, the current research status on plant tissue culture and regeneration, genetic transformation, existing problems, and industrialization application of the Dendrobium genus were reviewed.[Result] The tissue culture and regeneration system and industrialization application are nearly perfect for many Dendrobium species. However, there are still problems such as low transformation efficiency and difficulty in obtaining transgenic plants for most Dendrobium species.[Conclusion] Agrobacterium-mediated transformation method should be further improved for these Dendrobium species. The method should also be combined with the gene gun particle bombardment method. Other plant genetic transformation methods should be explored.

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备注/Memo

备注/Memo:
收稿日期:2020-04-11。
基金项目:国家自然科学基金项目(31560567);广西壮族自治区科技攻关项目(桂科攻1598006-3-11,桂科AB18294026);广西植物研究所基本业务费项目(桂植业15001,桂植业19001)资助。
作者简介:夏科(1985-),男,助理研究员,硕士。研究方向:药用植物栽培。Email:xiake4502@163.com。
通讯作者:仇硕(1977-),男,副研究员,博士。研究方向:观赏植物栽培生理及分子生物学。Email:qiushuo001@163.com。
更新日期/Last Update: 1900-01-01