[1]周建金,叶炜,邓才生,等.混倍体参薯再生植株的倍性与性状分析[J].亚热带农业研究,2021,17(01):16-21.[doi:10.13321/j.cnki.subtrop.agric.res.2021.01.004]
 ZHOU Jianjin,YE Wei,DENG Caisheng,et al.Analysis of ploidy and phenotypic traits of regenerated plants of mixoploids in Dioscorea alata Linn.[J].,2021,17(01):16-21.[doi:10.13321/j.cnki.subtrop.agric.res.2021.01.004]
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混倍体参薯再生植株的倍性与性状分析()
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《亚热带农业研究》[ISSN:1006-6977/CN:61-1281/TN]

卷:
17
期数:
2021年01期
页码:
16-21
栏目:
出版日期:
2021-05-15

文章信息/Info

Title:
Analysis of ploidy and phenotypic traits of regenerated plants of mixoploids in Dioscorea alata Linn.
作者:
周建金1 叶炜1 邓才生1 施小梅1 黄伟群2 华树妹1
1. 三明市农业科学研究院, 福建 沙县 365500;
2. 福建省种子总站, 福建 福州 350000
Author(s):
ZHOU Jianjin1 YE Wei1 DENG Caisheng1 SHI Xiaomei1 HUANG Weiqun2 HUA Shumei1
1. Institute of Agricultural Sciences of Sanming City, Shaxian, Fujian 365500, China;
2. Fujian General Station of Seeds, Fuzhou, Fujian 350000, China
关键词:
混倍体参薯组织培养倍性鉴定生物学性状
Keywords:
mixoploids in Dioscorea alata Linn.tissue cultureploidy identificationbiological characteristics
分类号:
S539
DOI:
10.13321/j.cnki.subtrop.agric.res.2021.01.004
摘要:
[目的] 通过组织培养,使四倍体和六倍体的混倍体参薯再生植株倍性和性状产生分离,以期获得倍性和性状均稳定的株系。[方法] 采用组织培养获得再生植株;通过染色体制片方法鉴定其倍性;再通过田间繁殖,鉴定再生植株染色体倍性和性状的稳定性。[结果] (1)2,4-D是诱导参薯愈伤组织形成的关键因子,愈伤组织诱导率达51.67%;椰子汁能有效促进愈伤组织的生长发育,分化率达46.67%。(2)混倍体参薯经组织培养后,再生植株出现了性状和倍性的分离。再生植株四倍体、六倍体和混倍体的获得率分别为28.33%、20.00%和56.67%。(3)单一倍性的再生植株经繁殖后,子代之间的生物学性状和染色体数目稳定一致。不同再生植株的染色体倍性和生物学性状有所差异。[结论] 利用组织培养分离纯化混倍体,是创建稳定植株和种质资源的有效方法。通过组织培养,从混倍体参薯中分离出多组生物学性状稳定的四倍体或六倍体株系,为参薯品种选育提供了新种质。
Abstract:
[Purpose] Through tissue culture, the tetraploid, hexaploid, and mixoploids in Dioscorea alata Linn. of regenerated plants were separated in order to obtain lines with stable ploidy and phenotypic trait. [Method] Tissue culture was used to obtain regenerated plants, the ploidy of the regenerated plants was identified by chromosome preparation method, and then the ploidy and trait stability of the regenerated plants were identified through field propagation. [Result] (1) 2,4-D was the key factor for inducing callus formation of D.alata. The callus induction rate was up to 51.67%. Coconut juice could effectively promote the growth and development of callus, with differentiation rate of up to 46.67%. (2) After tissue culture of mixoploids in D.alata, the regenerated plants separated for traits and ploidy. The acquisition rates of tetraploid, hexaploid and mixoploids in regenerated plants were 28.33%, 20.00%, and 56.67%, respectively. (3) Once the ploidy stable regenerated plants were reproduced and cultivated, their offspring showed stable and consistent biological characteristics and chromosome number. The chromosome ploidy and biological characteristics of different regenerated plants were different. [Conclusion] Using tissue culture to separate and purify mixoploids was an effective method to create stable plants and germplasm resources. Through tissue culture, this study isolated multiple sets of tetraploid or hexaploid strains with stable biological characteristics from D.alata, which provided new germplasm for the selection and breeding of D.alata.

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备注/Memo

备注/Memo:
收稿日期:2021-01-25。
基金项目:福建省科技厅资助项目(2019S0010)。
作者简介:周建金(1982-),女,助理研究员,硕士。研究方向:植物组织培养与育种。Email:55176609@qq.com。
通讯作者:华树妹(1977-),女,副研究员,硕士。研究方向:旱作植物育种。Email:hsm20082006@126.com。
更新日期/Last Update: 1900-01-01