[1]陈何,王晓,王乐,等.苋菜AtrNiR基因密码子偏好性与进化分析[J].亚热带农业研究,2021,17(01):48-56.[doi:10.13321/j.cnki.subtrop.agric.res.2021.01.009]
 CHEN He,WANG Xiao,WANG Le,et al.Codon preference and evolution analysis of AtrNiR gene in Amaranthus tricolor L.[J].,2021,17(01):48-56.[doi:10.13321/j.cnki.subtrop.agric.res.2021.01.009]
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苋菜AtrNiR基因密码子偏好性与进化分析()
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《亚热带农业研究》[ISSN:1006-6977/CN:61-1281/TN]

卷:
17
期数:
2021年01期
页码:
48-56
栏目:
出版日期:
2021-05-15

文章信息/Info

Title:
Codon preference and evolution analysis of AtrNiR gene in Amaranthus tricolor L.
作者:
陈何 王晓 王乐 赖钟雄 刘生财
福建农林大学园艺植物生物工程研究所, 福建 福州 350002
Author(s):
CHEN He WANG Xiao WANG Le LAI Zhongxiong LIU Shengcai
Institute of Horticultural Plant Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
关键词:
苋菜AtrNiR基因密码子偏好性进化分析
Keywords:
Amaranthus tricolor L.AtrNiR genecodon usage biasevolution analysis
分类号:
Q943.2S636.4
DOI:
10.13321/j.cnki.subtrop.agric.res.2021.01.009
摘要:
[目的] 为实现AtrNiR基因高效遗传转化提供依据。[方法] 从GenBank数据库和Codon Usage Database数据库获取24种植物的NiR基因序列和模式生物的基因组密码子偏好性数据。应用生物信息学软件分析苋菜AtrNiR基因密码子使用偏好性及进化特征。[结果] 苋菜AtrNiR基因的有效密码子数(ENc)为54.04,GC与GC3含量均低于50%,密码子偏好以A/T结尾;苋菜AtrNiR基因的密码子使用偏好性参数与石竹目物种相近;苋菜AtrNiR基因在ENc绘图中的基因位点更接近于期望曲线,说明其密码子选择偏好性在进化过程中受突变压力影响比较大;酵母菌表达系统适合苋菜AtrNiR基因在真核生物中异源表达,拟南芥、烟草、番茄、甜菜均可作为苋菜AtrNiR基因的遗传转化受体。[结论] 密码子偏好性分析结果对进一步开展AtrNiR转基因功能验证中受体物种选择、基因异源表达水平提升及植物遗传育种都有重要指导作用。
Abstract:
[Purpose] To provide a basis for high-efficient genetic transformation of AtrNiR gene. [Method] The genomic NiR sequence and codon preference data from 24 plant and model species were obtained from the GenBank and Codon Usage Database. The codon preference and evolutionary characteristics of amaranth AtrNiR gene were analyzed by bioinformatics software. [Result] The effective codon (ENc) value of amaranth AtrNiR gene was 54.04, the GC content and GC3 content were lower than 50%, and the amaranth AtrNiR gene codon preference ended with A/T. The codon preference parameters of AtrNiR gene were in accordance with Caryophyllales. The amaranth AtrNiR gene loci in the ENc mapping were closer to the expected curve, indicating that during the evolution process, some codons of the AtrNiR gene were preferentially selected under great influence by mutation pressure. The yeast expression system was more suitable for the heterologous expression of the AtrNiR gene and Arabidopsis thaliana, tobacco, tomato, and beet could be used as the genetic transformation host plants. [Conclusion] The results of codon preference analysis play an important role in guiding the further verification of AtrNiR transgene function in the selection of recipient species, the improvement of heterologous gene expression level, and the plant genetic breeding.

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备注/Memo

备注/Memo:
收稿日期:2021-01-22。
基金项目:福建省自然科学基金项目(2018J01700);福建农林大学科技创新专项(CXZX2017174,CXZX2016118,CXZX2017189)。
作者简介:陈何(1997-),女,硕士研究生。研究方向:蔬菜育种及生物技术。Email:2442369895@qq.com。
通讯作者:刘生财(1981-),副教授,硕士生导师。研究方向:园艺植物生物技术与品质代谢。Email:1215698900@qq.com。
更新日期/Last Update: 1900-01-01